The primary objective of this project was to develop a standard operating procedure for the use of swine oral fluids (SOF) as a diagnostic specimen for detection of endemic viral diseases such as Swine Influenza virus (SIV) and Porcine Reproductive Respiratory Disease virus (PRRSV) and foreign animal viral disease pathogens (FAD) such as African Swine Fever virus (ASFv), Classical Swine Fever virus (CSFv) and Foot and Mouth Disease (virus FMDv). For the execution of this research project viruses (BSL2 pathogens: SIV and PRRSV) and surrogate viral nucleic acids (NA) for ASFv. CSFv and FMDv) in varying concentrations were spiked into a collection of swine oral fluids to mimic what would appear in a clinical sample.